Astino Abbey (Italian: Monastero di Astino) is a monastery in the Astino Valley, Province of Bergamo, Lombardy, Italy. It is no longer active, and the buildings are currently in a state of profound decay.

Astino Abbey was founded around the year 1070 by a group of members of the Vallumbrosan Order led by John Gualbert during a time in which, through reforms, clerics were trying to revive the Catholic Church's position.
The Romanesque church and the first conventual buildings were built by Bertario, the first abbot, who supervised the abbey for 21 years until 1128.

The monastery was suppressed on 4 July 1797 by the ciil aurhorities of Bergamo. Its assets were given to the nearby hospital, founded and previously run by the monks.

In 1832 the site was put to use as a psychiatric hospital, which it remained until 1892. It was then used for agricultural purposes, and was sold to private buyers in 1923.

In 1973 the property was acquired by a private company for conversion into a golfing centre, but the plan ran into so much opposition that it never came to fruition, and the monastery buildings have been left neglected and falling into disrepair.

The Church of Santo Sepulchro ("the Holy Sepulchre") was consecrated in 1117 and remains. The building includes a cloister of the 15th century and a chapel to the memory of Blessed Guallo de Roniis, exiled bishop of Brescia.