The building, located within Villa Borghese near Piazza di Siena, was built at the end of the 18th century by Prince Marcantonio Borghese, both to satisfy his devotion to the Virgin Mary, and to offer his employees, the farmers, the opportunity to easily perform their religious practices.

The task of supervising the transformation work was entrusted to the architect Mario Asprucci, who decorated the small church with fine ornaments.

In 1829, Prince Camillo Borghese, on the occasion of the expansion and renovation of many of his possessions, commissioned the architect Canina to carry out the restoration and completion work on the small church that had become necessary due to the deterioration and removal of the ornaments previously made by Asprucci.

On that occasion, the small church was decorated with frescoes by the painter Pietro Carrarini, who at that time also created further works in other possessions of the Borghese family.

In 1959, the tomb of the sculptor Pietro Canonica, who lived for years in the nearby Fortezzuola (now a museum dedicated to him), was placed in the small church.